Knight was the son of physician Albion Williamson Knight and his second wife, the former Anna Bishop. He attended Central Grammar School and Duval High School in Jacksonville, Florida, then studied at Henry Frederick Hornbostel's Beaux-Arts styled "Atelier Hornbostel" in New York. He came to Birmingham in 1902 as a draftsman for Harry Wheelock. In 1906 he changed jobs and went to work for William Weston. From 1910 to 1913 he was in New York City, working for Clinton & Russell, and later for York & Sawyer. He returned to Birmingham as an associate of Weston's in 1914 and opened his own firm in 1916. A year later he partnered with William Warren to form Warren & Knight, Architects. That firm became Warren, Knight & Davis in 1922 with the addition of John Davis.
In addition to his work with the firm, Knight taught evening classes in architecture at the Birmingham YMCA and served as president of the Alabama Society of Architects in 1922. He was made a fellow of the American Institute of Architects in 1952.
- 1956 American Architects Directory (December 1955) American Institute of Architects/ R. R. Bowker
- Eugene Knight at Findagrave.com