Ebersole is one of four children born to Gary Lynn Ebersole and his wife, the former Noriko Yamazaki. He earned his bachelor's degree in anthropology, specializing in paleoanthropology, at St Lawrence University in Canton, New York. He conducted research in East Africa and later carried out contract archeology.
Ebersole came to Birmingham in 1999 to take over management of the fossil collections from the Red Mountain Museum after it was merged into the McWane Center. He has used the extensive collections to document numerous previously-unknown species and to shed light on the changing ecology of Alabama and the Gulf of Mexico over long time spans.
Ebersole also serves as an adjunct instructor at the UAB Department of Anthropology. In 2014 he led a group of archeologists documenting the historic Sloss Quarters for the Alabama Historical Commission.
- Harney, Erin E. & Jun A. Ebersole (2016) Gorgas House at the University of Alabama. Images of America Series. Arcadia Publishing. ISBN 9781467123846
- Pillion, Dennis (December 27, 2021) "Alabama paleontologist helps discover new 40 million-year-old shark species" The Birmingham News
- Jun Ebersole at scholar.google.com